DNA damage in human pulmonary cells exposed to organic extracts of PM2.5 collected from two urban sites at different traffic intensity

Genotoxic and oxidative effects of PM2.5 organic extracts from personal respirable dust samples of security guards working in two sites of Rome(Italy), were investigated. The PM 2.5 samples were collected at site 1 and site 2 characterized by moderate and high traffic intensity respectively.

The organic extracts were diluted at 0.10%, 0.15% and 0.25% and used to expose for 4h lung epithelial cells A549. Oxidative and direct DNA damage were evaluated by Fpg-comet assay analysing Tail moment values from fpg-enzyme treated cells (TMenz) and enzyme untreated cells (TM) respectively. Personal inhalation exposure to benzene, toluene and formaldehyde was monitored by passive sampling and GC/MS and HPLC/UV. Benzene, formaldehyde and particularly toluene levels were at site 2 higher than site 1. Comet results show a significant dose-dependent increase of TM and TMenz for site 2 and a lack of effect for site 1 indicating an induction of direct/oxidative DNA damage by urban PM 2.5 collected at site with high traffic intensity that seems to correlate with the pollution level indicated by the different amount of benzene and toluene known indicators of urban air pollution.



Copyright: © Aristotle University of Thessaloniki
Source: 2nd International Conference 2009 (Juni 2009)
Pages: 6
Price: € 5,00
Autor: Delia Cavallo
Cinzia Lucia Ursini
Renato Cabella
Andrea Gordiani
Raffaele Maiello

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