Chemical analysis of a large series of PAHs and POPs in airborne and sediment samples and their contribution to genotoxic, tumorpromoting and endocrine-disrupting effects

Combined chemical GC and HPLC analyses were successfully used to resolve several hundreds of individual aromatic compounds and different patterns of relative content of PAHs, methylated and nitrated PAH derivatives in various airborne and river sediment samples. Two approaches were used for sample fractionation. The fractionation on a silica column, based on analyte polarity, yielded aliphatic, nonpolar aromatic (containing both parental PAHs and POPs), H2SO4-silicatreated neutral POPs subfraction (in which PAHs were eliminated), semipolar aromatic (containing e.g. nitrated PAHs) and polar aromatic (with oxygenated PAHs, dialkyl phthalates and other, partly unidentified, polar aromatics) fractions.

As a second fractionation procedure, a multistep normalphase fractionation method was used for isolation of 18 fractions and separation of a smaller number of compounds. This enabled both chemical identification of minor, but highly toxic contaminants, and the assessment of relative contribution of potential toxicants to major toxic potencies as detected by specific in vitro bioassays (effect-directed analysis). AhR-mediated activity, determined by the DR-CALUX ® assay and RT-PCR detection of CYP1 induction, was found to be the most important toxic mode of action of PAHs present in both airborne and sediment extracts. Genotoxic effects (determined as DNA adducts formation, p53 phosphorylation and genotoxicity-induced apoptosis) were observable only at several orders of magnitude higher concentrations. Interestingly, a relatively high contribution of polar aromatic compounds (e.g. semipolar fraction of diesel exhaust particle extracts and highly polar aromatic fraction of river sediments) to AhR-mediated activity, and also to mild estrogenicity and anti-androgenicity was observed.



Copyright: © Aristotle University of Thessaloniki
Source: 2nd International Conference 2009 (Juni 2009)
Pages: 7
Price: € 5,00
Autor: M. Machala
M. Ciganek
Jiri Neca
Katerina Pencikova
Lenka Vykopalova

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