Biosorption of zinc from industrial wastewater using bacteria resistance

In this study in order to determine and investigate the effect of zinc concentration on bacteria resistance threshold, two samples of industrial wastewater(1 and 2) were collected. The concentrations of zinc in samples were 36 and 17 gL-1. In order to access heterotrophic plate count, wastewater samples were cultured on nutrient agar by pour plate method and to count and isolate the Zn resistant bacteria, samples were cultured by spread plate method on PHG-II agar include of 0.5 mML-1 of Zn in three dilution and two replications.

To assessment the resistance threshold in resistant bacteria, bacteria that were grown on PHG-II agar, were cultured on PHG-II with various concentrations ranging from 1 to 8 mML-1, respectively. According to the acquired results, microbial activity and viable population size(plate count), both decreased at the presence of Zn. There was a significant difference between the number of resistant bacteria in samples. It was found a significant correlation(r2=+0.99) between the viable population size and Zinc concentration. The average of MIC in the studied samples, were 2 and 1.46 mML-1, respectively. This study aimed to determine bacterial resistance threshold to Zn and investigation the relationship between the bacterial population size and Zn concentration in contaminated sites to assess bioremediation potential in these habitats. 

Copyright: © Aristotle University of Thessaloniki
Source: 2nd International Conference 2009 (Juni 2009)
Pages: 6
Price: € 5,00
Autor: Azadeh Nasr-e-Azadani
Dr. Arezoo Tahmourespour
Dr. Mehran Hoodaji
A. Abtahi

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